Abstract: This study was conducted to detection fungi associated with some spice were selling in local market such as Cumin, Anise, Cinnamon, Sesame, Fennel, Black pill, Nutmeg, Coriander, Black pepper, Turmeric, Ginger, Galangal, Caraway, Cardamom. Results were recorded twenty four fungus belonging to fifteen genus. The most predominant fungal genera encountered were Aspergillus spp, Penicillium spp, Alternaria spp, and Fusarium spp. Yeasts were also frequently recovered, but not identified. All fungi were isolated on Agar plate method than Blotter paper. The highly number of isolated fungi was recorded in Coriander but the lowest observed in Cumin.
Keywords: Spice, fungi, Plate Agar, Blotter paper, Libya.